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KMID : 0617220030140010103
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Duksung Bulletin Phamaceutical Sciences
2003 Volume.14 No. 1 p.103 ~ p.110
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Vanilloid Receptor -Independent Induction of Cancer Cell Apoptosis by Capsaicin
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Suh Yeon-Won
Lee Yong-Soo
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Abstract
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Effect of capsaicin, a vanilloid receptor (VR) agonist, on apoptotic cell death was investigated in HepG2 human hepatoblastoma cells. Capsaicin induced apoptosis in a dose-dependent manner evaluated by flow cytometric assay of hypodiploid nuclei stained with propidium iodide. The capsaicin-induced apoptosis was not altered by treatment a VR antagonist, capsazepine. In addition, capsaicin induced a rapid and sustained increase in intracellular Ca^(+2) concentration. This effect of capsaicin was significantly inhibited by BAPTA/AM, an intracellular Ca^(+2) chelator, but not changed by capsazepine. These results suggest that capsaicin induced apoptotic cell dealth through intracellular Ca^(+2) increase in human hepatoma cells, and that VRs may not be involved in the mechanism of the capsaicin-induced apoptosis.
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